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Smf app pcr 8009/22/2023 Upon DNA extraction, whole-genome or targeted bisulfite libraries are prepared and loaded on Illumina sequencers. ![]() DNA footprinting is performed by incubating permeabilized nuclei with recombinant methyltransferases. The technique combines the use of cytosine methyltransferases to footprint the genome with bisulfite sequencing to resolve transcription factor binding patterns at cis-regulatory elements. We recently developed single-molecule footprinting to simultaneously resolve the occupancy of multiple proteins including transcription factors, RNA polymerase II and nucleosomes on single DNA molecules genome-wide. ![]() ![]() Precise control of gene expression requires the coordinated action of multiple factors at cis-regulatory elements.
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